第 16 集: 阿冢與八冢對峙，銀時遭家臣重傷
2015年7月22日 播出
Overall episode #281 Asaemon points her blade at Yaemon of her own free will, but has a hard time due to Yaemon's overwhelming strength. Gintoki joins the fight but gets wounded by Hitotsubashi retainers backing Yaemon up. With Gintoki and Asaemon on the backfoot, Yaemon tells Asaemon about how Gintoki killed her biological father. This makes her waver, with sword in hand. Just who will Asaemon kill, Gintoki or Yaemon? (Source: Crunchyroll)