第 1 集: 504戰隊於威尼斯上空迎戰巨型Neuroi巢穴，Yoshika則隨Mio投入戰局。
Six months after the battle over Brittania, the 504th Joint Fighter Wing, while on a mission to make contact with the Neuroi, encounter an even greater Neuroi nest hovering over Venezia which obliterates the previous Neuroi and attacks relentlessly. Meanwhile, Yoshika, who had just graduated from middle school, receives a letter from Amaki Suwa, containing blueprints supposedly sent by her father. When Yoshika goes to deliver these to Mio, she gets word that the stronger Neuroi had overpowered the Strike Witches, aware that they are in danger. Yoshika is ordered by Mio to go home, but when she notices Mio heading into battle again, Yoshika takes her old Striker Unit and follows after her. Admiring her spirit, Mio allows Yoshika to accompany her. However, a week into their flight, their plane comes into contact with a Neuroi ship. (Source: Wikipedia)